FIGURE 2

The detection of key players in CBE treatment based on differential signaling network. (A) Predicted signaling transduction network inferred for CBE treatment. Red boxes indicate upregulation, and blue boxes indicate downregulation under CBE treatment compared to blank control. Key points are highlighted in yellow. (B) Changes in the transcriptional activity of NF-κB and AP-1 by BER, EVO, or CBE treatment. BEL-7402 cells were transfected with the reporter construct for 24 h and then treated with BER, EVO, or CBE for 12 h. For each sample, firefly luciferase activity is presented as normalized to Renilla luciferase activity. The results from each group are presented as the mean ± SD of at least three samples. The drug dose was equal to that used the in the RNA-seq experiment. Columns, mean; bars, SD (t-test; *p < 0.05; **p < 0.01).