HSPCs interact with IFI30+ cells in human fetal liver.a Double immunostaining against GILT/IFI30 and CD117 on sections of human fetal liver at different stages of gestation. b Distribution of GILT/IFI30+ cells (red line), CD117+ cells (brown line), and IFI30+ cells in contact with CD117+ cells (green line). For each developmental stage, only one human fetal liver sample was used (N = 1), but three different sections were analyzed. c High magnification of double immunostaining against GILT/IFI30 (red) CD117 (brown) and nuclei (blue), on sections of human fetal liver at 13 weeks of gestation. Scale bars: 10 μm. d Double immunostaining against GILT/IFI30 (black), CD68 (cyan), and nuclei (blue) in sections of human fetal liver at 13 weeks of gestation. All red arrows point to double-stained cells. Scale bars: 100 μm (a); 25 μm (c); 200 μm. Right panel shows a magnification of the region delimited by the red dotted line (d).
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