Knockdown of ep300a and plaub caused impaired intersegmental vessel (ISV) formation in zebrafish. Lateral views of the trunk of Tg(flk:gfp) zebrafish larvae (dorsal or ‘to the top’ and anterior or ‘to the left’) at 3 days post-fertilization (dpf) (A–H). Arrows indicate ISVs with abnormal branch formation. Arrowheads indicate ISVs with abnormal shape. Quantification of the number of embryos with ISV defects (I) in the control, ep300a, ep300b, plaua, and plaub morpholino (MO)-injected larvae at 3 dpf ([Control MO], normal: 97.43%, less: 2.57%, severe: 0% (n = 34); [ep300a MO], normal: 0% less: 40.27%, severe: 59.73%, (n = 37); [ep300b MO], normal: 43.25%, less: 56.75%, severe: 0%, (n = 37); [plaua MO], normal: 90.97%, less: 9.03%, severe: 0%, (n = 36); [plaub MO], normal: 11.43%, less: 8.57%, severe: 80%, (n = 35); [ep300a/plaub MO], normal: 0%, less: 5.41%, severe: 94.59%, (n = 37)). Quantification of defective ISVs (J) in the control, ep300a, ep300b, plaua, and plaub MO-injected larvae and ep300a MO + RNA and plaub MO + RNA-injected larvae at 3 dpf (Control MO: 0.72 ± 0.27 (n = 11), ep300a MO: 6.62 ± 0.49 (n = 13), ep300a MO + RNA: 1.9 ± 0.43 (n = 10), ep300b MO: 2.54 ± 0.27 (n = 13), plaua MO: 1.39 ± 0.24 (n = 13), plaub MO: 5.83 ± 0.65 (n = 12), plaub MO + RNA: 0.9 ± 0.18 (n = 10), ep300a/plaub MO: 9.15 ± 0.73 (n = 13). **p < 0.01, ***p < 0.01, ****p < 0.0001; scale bar = 100 µm).
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