FIGURE

Fig. 5

ID

ZDB-FIG-210712-5

Publication

Butti et al., 2021 - Reduced C9orf72 function leads to defective synaptic vesicle release and neuromuscular dysfunction in zebrafish

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Fig. 5

Adult zebrafish C9-miR display muscle atrophy, smaller motoneurons and behavioural deficits.

a Representative traces of swimming activity of five adult controls and C9-miR fishes (12-month old) during 30 s (left panel). b C9-miR fish exhibit behavioural deficits (right panel) (n = 5, ***p < 0.0001, Student’s t test). c Adult 12-month-old NMJs were examined in trunk section by co-immunostaining SV2 (green) and α-bungarotoxin (red). d Quantification of the colocalizing presynaptic and postsynaptic clusters at NMJ in adult (12-month old) wild-type control (n = 6) and C9-miR fish (n = 7) (***p < 0.0001, Student’s t test). e ChAT staining in adult zebrafish spinal cord . f Large (mature) motor neurons (inset in e; scale bar = 10 μm) are reduced in size in C9-miR compared to controls (n = 4; ***p < 0.0001, Student’s t test). g Examination of adult zebrafish muscle myotomes by haematoxylin and eosin staining. h C9-miR fish display a smaller diameter of muscle fibres compared to controls (n = 10; ***p < 0.0001, Student’s t test). Data are presented as mean ± SEM. Scale bar = 50 μm. n represents the number of sections from three adult fish per genotype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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