ZFIN ID: ZDB-FIG-210707-68
Ghilardi et al., 2021 - Inner Ear and Muscle Developmental Defects in Smpx-Deficient Zebrafish Embryos. International Journal of Molecular Sciences   22(12) Full text @ Int. J. Mol. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Protruding-mouth to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Figure 1 Phenotypical and ear defects in smpx zebrafish morphants (MO). (A,B) representative phenotype caused by the lack of Smpx. Control-MO (A) and smpx-MO (B) injected embryos. The red arrow points to the downward trunk/tail curvature indicative of cilia disfunction. (C–F) kinocilia of the anterior (ac), lateral (lc) and posterior (pc) cristae of the inner ear of 72 hpf (C,D) and 5 dpf (E,F) larvae injected with ctrl- (C,E) and smpx-MO (D,F). Cristae kinocilia are stained with the antibody against acetylated tubulin (ac tub); phalloidin is used to label the actin (act) of the stereocilia. White arrowheads indicate the different ‘posture’ of the lateral crista kinocilia. (G,H) SEM images of the ciliary bundle in the control larvae (G) and smpx morphants (H). The arrowhead indicates the kinocilium, with the arrow the stereocilia. (I,J) representative confocal images of the otic cavity injected with FM4-64, labeling the inner ear hair cells in the control larvae ((I), n = 4) and smpx morphants ((J), n = 7). The asterisks indicate the lack of signal in the ac, lc and pc of the Smpx-deficient larvae, suggestive of an impaired mechanotransduction. (I’) magnified view of the anterior crista in (I) (white rectangle). Scale bars = 50 μm in (C–F); 25 μm in (I,J); 1 μm in (G,H).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC AB control Protruding-mouth anterior crista kinocilium
IHC Protruding-mouth lateral crista kinocilium
IHC Protruding-mouth posterior crista kinocilium
IHC Day 5 anterior crista kinocilium
IHC Day 5 lateral crista kinocilium
IHC Day 5 posterior crista kinocilium
IHC AB + MO1-smpx control Protruding-mouth anterior crista kinocilium
IHC Protruding-mouth lateral crista kinocilium
IHC Protruding-mouth posterior crista kinocilium
IHC Day 5 anterior crista kinocilium
IHC Day 5 lateral crista kinocilium
IHC Day 5 posterior crista kinocilium
IHC AB + MO2-smpx control Protruding-mouth anterior crista kinocilium
IHC Protruding-mouth lateral crista kinocilium
IHC Protruding-mouth posterior crista kinocilium
IHC Day 5 anterior crista kinocilium
IHC Day 5 lateral crista kinocilium
IHC Day 5 posterior crista kinocilium
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-smpx control Long-pec post-vent region curved ventral, abnormal
Long-pec trunk curved ventral, abnormal
Protruding-mouth lateral crista kinocilium decreased length, abnormal
Protruding-mouth lateral crista kinocilium increased length, abnormal
Protruding-mouth lateral crista kinocilium straight, abnormal
Protruding-mouth lateral crista stereocilium shortened, abnormal
Day 5 anterior crista hair cell non-functional, abnormal
Day 5 inner ear mechanosensory behavior decreased process quality, abnormal
Day 5 lateral crista hair cell non-functional, abnormal
Day 5 posterior crista hair cell non-functional, abnormal
AB + MO2-smpx control Long-pec post-vent region curved ventral, abnormal
Long-pec trunk curved ventral, abnormal
Protruding-mouth lateral crista kinocilium decreased length, abnormal
Protruding-mouth lateral crista kinocilium increased length, abnormal
Protruding-mouth lateral crista kinocilium straight, abnormal
Protruding-mouth lateral crista stereocilium shortened, abnormal
Day 5 anterior crista hair cell non-functional, abnormal
Day 5 inner ear mechanosensory behavior decreased process quality, abnormal
Day 5 lateral crista hair cell non-functional, abnormal
Day 5 posterior crista hair cell non-functional, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.