Inhibition of VEGFRs with sunitinib reduces endothelial remodeling in zebrafish embryos. (A) Experimental setup used: zebrafish are imaged at 3 hpi (hours post-injection). (B) Quantification of intravascular, remodeling and extravasated cells 3hpi. N cells: vehicle = 622, sunitinib 2 µM = 375, sunitinib 5 µM = 254. N embryos : vehicle = 62, sunitinib 2 µM = 34, sunitinib 5 µM = 27, Kruskal–Wallis test followed by Dunn's multiple Comparison test. (C) Representative image of arrested D2A1 cells in the caudal plexus of vehicle or sunitinib treated embryos. Vehicle: DMSO. (D) Representative confocal image of the caudal plexus architecture (upper panel) and blood flow (lower panel, RBC = red blood cells) 3 h post-treatment with sunitinib vs vehicle in Tg(fli1a:EGFP;gata1:DsRed). (E) Quantification of the effect of sunitinib on vessel architecture after 3 h of treatment with vehicle or 5 µM of sunitinib, N = 8, Student t test. (F) Blood flow perfusion profiles of the same embryos (from D) and quantification of the blood flow velocity in the caudal plexus of embryos in both conditions after 3 h of treatment with vehicle or 5 µM of sunitinib. N embryos: vehicle = 8, sunitinib 5 µM = 10. Mann Whitney test.
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