FIGURE

Figure 1

ID

ZDB-FIG-210630-41

Publication

Jerafi-Vider et al., 2021 - VEGFC/FLT4-induced cell-cycle arrest mediates sprouting and differentiation of venous and lymphatic endothelial cells

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Endothelial cells sprout from the PCV in G1 phase

(A) Schematic representation of cell cycle stages in ECs as highlighted by the Tg(fli1a:Gal4FF;UAS:FUCCI) reporter.

(B–E) Selected confocal snapshots from a time-lapse series of a Tg(fli1a:Gal4FF;UAS:FUCCI;kdrl:tagBFP) embryo depicting the distribution of late G1 (red) ECs in the PCV (outlined by dashed lines) at 26 (B and C, light-blue arrowheads) and 42 (D and E, light-blue arrowheads) hpf.

(F) Fraction of late-G1 ECs out of total PCV cells (n_{26 hpf}_embryos = 11; n₄₂_{hpf embryos} =9 ). Data show mean ± SEM (unpaired t test).

(G) Spatial distribution of late-G1 ECs in the PCV at 26 and 42 hpf (n_{26 hpf embryos} = 11; n_{42 hpf embryos} =9 ). Data show mean ± SEM (unpaired t test).

(H–O) Selected snapshots from a time-lapse series of a Tg(fli1a:Gal4FF;UAS:FUCCI;UAS:Kaede) embryo showing two different ECs budding off the PCV in late-G1 stage (H and L, white arrowheads) and re-entering cell cycle upon crossing the DA (K and N, purple arrowhead).

(P–S) Confocal images of 30 (P and R) and 48 (Q and S) hpf Tg(fli1:EGFP) embryos immuno-stained with p53 (P and Q, light-blue arrowheads) or p27 (R and S, light-blue arrowheads) antibodies. Co-localization channel is shown in yellow, and PCV is outlined by dashed lines.

(T and U) Spatial distribution of p53+ (n_{30 hpf} = 7, n_{48 hpf} = 4) (T) and p27+ (n_{30 hpf} = 18, n_{48 hpf} = 8) (U) ECs in the PCV. Data show mean ± SEM (one-way ANOVA plus Tukey`s post-hoc test). Scale bars: 40 μm (B–E and H–O), 70 μm (P–S); ^∗p < 0.05, ^∗∗∗p < 0.001, ^∗∗∗∗p < 0.0001; ns, not significant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.