FIGURE

Fig. 1

ID

ZDB-FIG-210603-18

Publication

Zhou et al., 2020 - The multifaceted role of protein kinase CK2 in high-risk acute lymphoblastic leukemia

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Fig. 1

Overexpression of wild-type but not enzyme dead CK2α promotes the onset of MYC-induced acute lymphoblastic leukemia in zebrafish. (A) Diagram of the experimental design. (B) Thymic fluorescence in the Tg(rag2:MYC-ER;lck:EGFP) (left), Tg(rag2:MYC-ER;lck:EGFP;lck:CK2αwt; rag2:mCherry) (middle), and Tg(rag2:MYC-ER;lck:EGFP;lck:CK2αK68M;rag2:mCherry) (right) fish raised in 129 nM 4-hydroxytamoxifen (4HT) at 12 weeks of life. One representative fish is shown for each group. (C) Kaplan-Meier analysis of tumor-free fish revealed that overexpression of CK2αwt but not CK2αK68M significantly accelerated the onset of MYC-induced acute lymphoblastic leukemia (ALL) (P=0.0013 for Tg(rag2:MYC-ER;lck:EGFP) [MYC-ER; green line] vs. Tg(rag2:MYCER; lck:EGFP;lck:CK2αwt; rag2:mCherry); [MYC-ER;CK2αwt; red line] n=19 and n=22, respectively; and P=0.0008 for MYC-ER;CK2αwt [red line] vs.Tg(rag2:MYCER; lck:EGFP;lck:CK2αK68M;rag2:mCherry) [MYC-ER;CK2αK68M; black line], n=22 and n=13, respectively). There was no statistical significance between MYCER and MYC-ER;CK2αK68M fish. Statistical analysis was performed using the log-rank test. The scale bar in the left and middle panel of Figure 1B =1 mm and in the right panel =200 mm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cz2Tg; zdf14Tg bu10Tg; cz2Tg; zdf14Tg bu12Tg; cz2Tg; zdf14Tg
Observed In:	thymus neoplasm
Stage:	Days 30-44

Phenotype Detail

Acknowledgments

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