Knocking out of claudin h caused the defects of otic vesicle and impaired the development of cristae hair cells and neuromast hair cells. (A) Imaging analysis of otic vesicle and cristae hair cells in control and claudin h mutants at 96 hpf. The yellow dotted line marked the boundary of the otic vesicle. (B) The statistical analysis of otic vesicle area in control and claudin h mutants at 96 hpf. (C,D) The statistical analysis of the number of different cristae hair cells and the cilia lengths in control and claudin h mutants at 96 hpf. (E) The imaging analysis of control and claudin h mutants at 96 hpf in bright field and fluorescent field. (F) Confocal imaging analysis of L1 hair cell clusters in the posterior lateral line of control and claudin h mutants at 96 hpf. (G,H) Quantification of the number of hair cell clusters and the number of hair cells per L1 neuromast in the posterior lateral line of control and claudin h mutants at 96 hpf. Values with *, ***, and ****above the bars are significantly different (P < 0.05, P < 0.001, and P < 0.0001, respectively).
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