Figure 4—figure supplement 1.

(A) Rows: linked cell types. Schistosome cell types correspond to Leiden clusters. Columns: genes linked by SAMap with overlapping eukaryotic eggNOG orthology groups. We calculate the average standardized expression of each gene in an orthology group for its corresponding cell type in a particular pair and report the highest expression. A selected set of orthology groups corresponding to transcriptional regulators are labeled. (B) Fluorescence in situ hybridization shows the co-expression of wnt11 (Smp_156540) and a panel of muscle markers (collagen, troponin, myosin and tropomyosin) in S. mansoni juveniles. The body wall muscles are expected to be located close to the parasite surface (dashed outline). The images are maximum intensity projections constructed from ~10 confocal slices with optimal axial spacing recommended by the Zen software collected on a Zeiss LSM 800 confocal microscope using a 40× (N.A. = 1.1, working distance = 0.62 mm) water-immersion objective (LD C-Apochromat Corr M27). (C) Whole mount in situ hybridization images showing that the expression of wnt11 and frizzled (Smp_174350) are concentrated in the parasite tail (arrows) with decreasing gradients extending anteriorly. In planarian muscles, Wnt genes provide the positional cues for setting up the body plan during regeneration (Scimone et al., 2017; Reddien, 2018). The presence of an anterior-posterior expression gradient of wnt11 and frizzled in muscles of schistosome juveniles suggests that they may have similar functional roles in patterning during development.