FIGURE

Fig. 2

ID

ZDB-FIG-210519-17

Publication

Zhang et al., 2021 - Rational construction of a reversible arylazo-based NIR probe for cycling hypoxia imaging in vivo

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Fig. 2

The reversible reaction of HDSF in hypoxia-reoxygenation cycle.

ESI-MS spectra of probe solutions (20 μM in PBS buffer) incubated with RLM (250 μg mL⁻¹) and NADPH (100 μM), a HDSF incubated in hypoxic condition. The supernatant of the reaction solution was directly used for detection. b HDSF solution exposed in the air after incubated in the hypoxic environment. c HDMA incubated in hypoxic condition. Solutions of (b) and (c) were quenched with MeCN, then vortexed and centrifuged, and the organic layers were used for detection respectively.

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Antibody Labeling

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