Fig. 6

Inhibition of monocarboxylate transport prevents downregulation of glucose (Gluc) carriers. Although environmental glucose at concentrations between 5 and 20 mM had no clear effect, inhibition of SLC16 family members by BAY 8002 (BAY) at 5 µM resulted in a significant upregulation of slc2a1a. This upregulation was ameliorated by the addition of glucose in a dose-dependent fashion, suggesting that blocking the ability to use monocarboxylates as substrates augments the reliance on glucose as substrate for tissue homeostasis. A: representative images of whole-mount in situ hybridization (WISH) for slc2a1a under the indicated conditions. B: quantification of the WISH data shown in A. The numbers above the bars indicate the counts of analyzed embryos. Significance was calculated using a two-sided t test. C: quantitative RT-PCR for slc2a1a of embryos treated with BAY 8002 or BAY 8002 + glucose confirmed the findings from the WISH experiments: the upregulated expression of slc2a1a in the presence of BAY 8002 was normalized by the addition of glucose. P values were calculated using a Student?s t test. Gluc, glucose; SLC, solute carrier; WISH, whole-mount in situ hybridization.