FIGURE

Figure 1—figure supplement 2.

ID

ZDB-FIG-210514-28

Publication

Yi et al., 2021 - A calcineurin-mediated scaling mechanism that controls a K⁺-leak channel to regulate morphogen and growth factor transcription

Other Figures

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Figure 1—figure supplement 2.

Protein expression of selected developmental genes and β-catenin subcellular distribution.

(A) Example blot for Shh expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (B) Example blot for Lef1 expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (C) Example blot for β-catenin expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (D) Example blot for β-actin expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (E–G) Three adjacent serial confocal sections through the fin of a control non-heat-shocked Tg[hsp70:kcnk5b-GFP] fish. Dapi stains the nuclei (blue) and immunohistochemistry for β-catenin (red). (a) Lower magnifications. (b) Corresponding higher magnifications for each preceding (a) panel. (H–J) Three adjacent serial confocal sections through the fin of a heat-shocked Tg[hsp70:kcnk5b-GFP] fish. Dapi stains the nuclei (blue) and immunohistochemistry for β-catenin (red). (a) Lower magnifications. (b) Corresponding higher magnifications for each preceding (a) panel. (K) Graphed measurements of β-catenin fluorescence intensity of stained sections. All scale bars are equal to 10 μm. The data for each panel represent three or more experiments. The graphed data points show all technical replicates.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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