Figure 1—figure supplement 2.

Figure 1—figure supplement 2.

(A) Example blot for Shh expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (B) Example blot for Lef1 expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (C) Example blot for β-catenin expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (D) Example blot for β-actin expression in adult fins with or without heat-shock induction of the Tg[hsp70:kcnk5b-GFP] transgene. (E–G) Three adjacent serial confocal sections through the fin of a control non-heat-shocked Tg[hsp70:kcnk5b-GFP] fish. Dapi stains the nuclei (blue) and immunohistochemistry for β-catenin (red). (a) Lower magnifications. (b) Corresponding higher magnifications for each preceding (a) panel. (H–J) Three adjacent serial confocal sections through the fin of a heat-shocked Tg[hsp70:kcnk5b-GFP] fish. Dapi stains the nuclei (blue) and immunohistochemistry for β-catenin (red). (a) Lower magnifications. (b) Corresponding higher magnifications for each preceding (a) panel. (K) Graphed measurements of β-catenin fluorescence intensity of stained sections. All scale bars are equal to 10 μm. The data for each panel represent three or more experiments. The graphed data points show all technical replicates.