FIGURE

Fig. 6

ID

ZDB-FIG-210501-16

Publication

Blokzijl-Franke et al., 2021 - Phosphatidylinositol-3 kinase signaling controls survival and stemness of hematopoietic stem and progenitor cells

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Fig. 6

ScRNA-seq seq reveals a shift toward HSPCs in LY294002-treated 5 dpf old embryos.

CHTs of control and LY294002-treated embryos (5 dpf, ~100 embryos each) were dissected, pooled, dissociated, FACS sorted and submitted to SORT-seq. a Visualization of k-medoid clustering and cell-to-cell distances using t-SNEs. Each dot represents a single cell. Colors and numbers indicate cluster and correspond to colors in h. In total, 684 cells are shown. b–f t-SNEs maps highlighting the expression of marker genes for each of the different cell types found. Transcript counts are given in a linear scale. b Erythrocyte progenitors, c Thrombocyte/erythrocyte progenitors, d HSPCs, e Myeloid progenitors, f Neutrophil progenitors. g t-SNE map highlighting the distribution of LY294002-treated embryos and their controls (h). The percentage of cells from LY294002-treated embryos and their controls in the different clusters. Fisher’s exact test with multiple testing correction (Fdr) were used for statistical analysis. **p < 0.01, ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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