FIGURE

Fig. 2

ID

ZDB-FIG-210501-12

Publication

Blokzijl-Franke et al., 2021 - Phosphatidylinositol-3 kinase signaling controls survival and stemness of hematopoietic stem and progenitor cells

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Less HSPCs colonize the CHT in <italic>ptena</italic><sup><italic>−/−</italic></sup><italic>ptenb</italic><sup><italic>−/−</italic></sup> mutant embryos than in wild-type.

a The VDA of tg(kdrl:Dendra2) was photoconverted green-to-red at 26–28 hpf. By 50–60 hpf red HSPCs derived from the photoconverted VDA had colonized the CHT in b sibling and cptena^−/−ptenb^−/− larvae. d The number of GFP^low HSPCs at 48 hpf in the CHT of tg(cd41:eGFP) siblings (sib) (n = 25) (e) and ptena^−/−ptenb^−/− mutants (mut) (n = 12) (f) is expressed as average number of cells in siblings (n = 41) or ptena^−/−ptenb^−/−. Mutants (n = 18) (g) is expressed as average number of cells in siblings (n = 33) or ptena^−/−ptenb^−/− mutants after injection with synthetic ptenb-mRNA (n = 15). Error bars indicate standard error or the mean (SEM). Shapiro–Wilk test for normal distribution and Welch’s two-tailed t-test were used for statistical analysis; ***p < 0.001. Representative embryos are shown and the number of embryos that showed this pattern/total number of embryos is indicated.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Oncogene