FIGURE

Figure 2

ID

ZDB-FIG-210424-2

Publication

Inoue et al., 2021 - Leucyl-tRNA synthetase deficiency systemically induces excessive autophagy in zebrafish

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Figure 2

Larsb-knockout larvae display severe developmental phenotype and liver abnormality with early lethality. (A) Bright field lateral views of larsb^+/+ and larsb^−/− embryos at 96 h post fertilization (hpf). Scale bar: 500 μm (top row) and 300 μm (bottom row). (B) Kaplan–Meier survival curve of larsb^+/+ (n = 32), larsb^+/- (n = 63), and larsb^−/− (n = 23) larvae. (C) Lateral views of larsb^+/+ and larsb^−/− embryos containing haemoglobin-containing cells (white arrows) stained with o-dianisidine at 72 hpf. (D) Morphological abnormality at 3 dpf and 6 dpf in the livers of larsb^−/− larvae under Tg[fabp10:mcherry] background. Scale bar: 300 μm. (E) Quantification of liver size in larsb^−/− larvae under Tg[fabp10:mcherry] background (3 dpf and 6 dpf). Liver sizes were evaluated using ImageJ software version 1.52a (https://imagej.nih.gov/ij/). n = 5 fish/group. Error bars indicate SEM. Student’s t-test; ***P < 0.001. Statistics were calculated and the figure was produced in GraphPad software version 8 (https://www.graphpad.com/scientific-software/prism/). Larsb: leucyl-tRNA synthetase b, dpf: days post fertilization.

Expression Data

Gene:	mCherry
Fish:	oi2Tg (AB) lars1b^oi3/oi3; oi2Tg (AB)
Anatomical Term:	liver
Stage Range:	Protruding-mouth to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	lars1b^oi3/oi3 (AB) lars1b^oi3/+ (AB) oi2Tg (AB) lars1b^oi3/oi3; oi2Tg (AB)
Observed In:	liver nucleate erythrocyte pericardium swim bladder whole organism
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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