FIGURE

Figure 1

ID

ZDB-FIG-210417-8

Publication

Magnani et al., 2021 - uhrf1 and dnmt1 Loss Induces an Immune Response in Zebrafish Livers Due to Viral Mimicry by Transposable Elements

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Figure 1

uhrf1 and dnmt1 loss causes DNA methylation loss in zebrafish livers. (A) Slot blot of genomic DNA extracted from pools of 5 dpf uhrf1^−/− and dnmt1^−/− mutant and WT siblings livers. (B) Quantification of 5-MeC measured by slot blot normalized to double stranded DNA (dsDNA). Each dot represents one clutch. *p < 0.05 and **p < 0.005 calculated by unpaired t-test. (C) Time course imaging of live larvae by fluorescent stereoscope microscopy. Tg(c269°ff ; 10XUAS:dsRed; fabp10a:GAL4; cmlc2:EGFP);dnmt1−/− and Tg(c269°ff; 10XUAS:dsRed; fabp10a:GAL4; cmlc2:EGFP);uhrf1−/− are shown at each age, showing the liver (dsRed), the heart (GFP) as a marker of transgenesis and, in green, the liver with DNA hypomethylation in mutants only. Images are representative of 100% of larvae from 3 clutches observed for each genotype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol