FIGURE

Fig 4

ID

ZDB-FIG-210331-32

Publication

McGowan et al., 2021 - Wnt16 Elicits a Protective Effect Against Fractures and Supports Bone Repair in Zebrafish

Other Figures

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Fig 4

TRAP⁺ punctae accumulate near to fractures in wnt16^−/− zebrafish postinjury. Fins from WT and wnt16^−/− zebrafish were amputated at 0 hours postfracture (hpi), 24 hpi, 4 days postfracture (dpi) and 7 dpi before undergoing staining to detect the presence of tartrate‐resistant acid phosphatase (TRAP). (A) Representative images of fractures stained for TRAP. Scale bar = 100 μm. (B) Overall coverage of TRAP was measured by calculating the total % area stained within 300 μm of the fracture site. No significant difference in the amount of TRAP⁺ stained area between WT and wnt16^−/− fractures was found. (C) The number of TRAP⁺ punctae present within 300 μm of the fracture site were quantified and showed a significantly higher number of punctae at 24 hpi and 4 dpi in the fractures of wnt16^−/− zebrafish compared with WT. *p < 0.05; N ≥ 6 per genotype.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	wnt16^{bsl451/bsl451}; gl23Tg wnt16^{bsl667/bsl667}; gl23Tg
Condition:	compression injury: caudal fin
Observed In:	caudal fin principal ray osteoclast osteoclast acid phosphatase activity
Stage:	Adult

Phenotype Detail

Acknowledgments

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