Fig 4

Fig 4

TRAP⁺ punctae accumulate near to fractures in wnt16^−/− zebrafish postinjury. Fins from WT and wnt16^−/− zebrafish were amputated at 0 hours postfracture (hpi), 24 hpi, 4 days postfracture (dpi) and 7 dpi before undergoing staining to detect the presence of tartrate‐resistant acid phosphatase (TRAP). (A) Representative images of fractures stained for TRAP. Scale bar = 100 μm. (B) Overall coverage of TRAP was measured by calculating the total % area stained within 300 μm of the fracture site. No significant difference in the amount of TRAP⁺ stained area between WT and wnt16^−/− fractures was found. (C) The number of TRAP⁺ punctae present within 300 μm of the fracture site were quantified and showed a significantly higher number of punctae at 24 hpi and 4 dpi in the fractures of wnt16^−/− zebrafish compared with WT. *p < 0.05; N ≥ 6 per genotype.