Fig. 5

Endochondral growth arrest in adult zebrafish. A, Anatomy of one‐year old adult zebrafish cartilage‐derived pharyngeal skeleton with alizarin red stained bone and alcian blue stained cartilage. Frames indicate regions highlighted in panels B to K. B, Alcian blue stained PQ synchondrosis flanked by alizarin red stained QA and MP bones. Chondrocytes are absent from the QA and MP, as shown in higher magnification inset. C, Bone matrix of the QA and MP flanking the PQ synchondrosis is stained by both alizarin red and calcein green. D, Alcian blue stained MC flanked posteriorly by alizarin red stained AR bone. E, Bone matrix of the AR flanking MC is stained by both alizarin red and calcein green. New bone growth on DE is stained by alizarin red alone (double‐arrow and *). F, Alcian blue stained HS synchondrosis flanked by alizarin red stained SY and HM bones. Chondrocytes are absent from the SY and HM, as shown in higher magnification inset. G, Bone matrix of the SY and HM flanking the HS synchondrosis is stained with both alizarin red and calcein green. H, Alcian blue stained dorsal CH synchondrosis flanked by alizarin red stained CH and EH bones. Chondrocytes are absent from the CH and EH. I, Bone matrix of the CH and EH flanking the dorsal CH synchondrosis is stained by both alizarin red and calcein green. J, Alcian blue stained CB1 distal cartilage flanked by alizarin red stained CB1 bone. Chondrocytes are absent from the CB1. K, Bone matrix of the CB1 flanking the distal cartilage is stained by both alizarin red and calcein green. Scale bar: 640 μm. AR, articular; BB1, basibranchial 1; CB1, ceratobranchial 1; CH, ceratohyal; DE, dentary; EH, epihyal; HM, hyomandibula; HS, hyosymplectic; MC, Meckel's cartilage; MP, metapterygoid; PQ, palatoquadrate; QA, quadrate; SY, symplectic