PUBLICATION
Endochondral growth zone pattern and activity in the zebrafish pharyngeal skeleton
- Authors
- Heubel, B.P., Bredesen, C.A., Schilling, T.F., Le Pabic, P.
- ID
- ZDB-PUB-200828-30
- Date
- 2020
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 250(1): 74-87 (Journal)
- Registered Authors
- Schilling, Tom
- Keywords
- bone, cartilage, hypertrophy, indeterminate, metamorphosis, postembryonic
- MeSH Terms
-
- Animals
- Bone Development*
- Chondrocytes/physiology
- Pharynx/growth & development
- Skeleton/growth & development*
- Zebrafish/growth & development*
- PubMed
- 32852849 Full text @ Dev. Dyn.
Citation
Heubel, B.P., Bredesen, C.A., Schilling, T.F., Le Pabic, P. (2020) Endochondral growth zone pattern and activity in the zebrafish pharyngeal skeleton. Developmental Dynamics : an official publication of the American Association of Anatomists. 250(1):74-87.
Abstract
Background Endochondral ossification is a major bone forming mechanism in vertebrates, defects in which can result in skeletal dysplasia or craniofacial anomalies in humans. The zebrafish holds great potential to advance our understanding of endochondral growth zone development and genetics, yet several important aspects of its biology remain unexplored. Here we provide a comprehensive description of endochondral growth zones in the pharyngeal skeleton, including their developmental progression, cellular activity, and adult fates.
Results Postembryonic growth of the pharyngeal skeleton is supported by endochondral growth zones located either at skeletal epiphyses or synchondroses. Col2a1a and col10a1a in situ hybridization and anti-PCNA immunostaining identify resting-, hypertrophic- and proliferative zones, respectively, in pharyngeal synchondroses. Cellular hypertrophy and matrix deposition contribute little, if at all, to axial growth in most skeletal elements. Zebrafish endochondral growth zones develop during metamorphosis and arrest in adults.
Conclusions Two endochondral growth zone configurations in the zebrafish pharyngeal skeleton produce either unidirectional (epiphyses) or bidirectional (synchondroses) growth. Cell proliferation drives endochondral growth and its modulation, in contrast to mammalian long bones in which bone length depends more on cell enlargement during hypertrophy and intramembranous ossification is the default mechanism of bone growth in zebrafish adults. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping