Figure 8

HDL injection did not impact microglia recruitment and injury-induced proliferation at the ventricular zone after telencephalic injury. Zebrafish underwent stab wound injury of the telencephalon and were injected with 80 mg/kg of plasma HDL (n = 9) or PBS as a control (n = 10) and sacrificed at 2- or 5-days post lesion (dpl). (A) l-Plastin immunohistochemistry (green) showing increased microglial recruitment in the injured telencephalon at 2 dpl. (B) Quantification of the l-Plastin-positive area in the contralateral and injured hemisphere in PBS-injected fish, demonstrating significant upregulation of microglia recruitment following brain injury at 2 dpl. (C) l-Plastin fold induction between lesioned and non-lesioned hemispheres in HDL and PBS-injected fish at 2 dpl. (D) PCNA immunohistochemistry (red) showing increased ventricular proliferation in the injured telencephalon at 5 dpl. (E) Quantification of the PCNA-positive area in the contralateral and injured hemisphere in PBS-injected fish, demonstrating a significant upregulation of proliferation following brain injury at 5 dpl. (F) PCNA fold induction between lesioned and non-lesioned hemispheres in HDL and PBS injected fish at 5 dpl. **p < 0.01. Scale bar = 100 μm.