Figure 7

Intracerebroventricular injection of HDLs results in their uptake by neural stem cells but did not modify their proliferation. (A) Overview pictures of GFAP::GFP (green) zebrafish heads following injection with fluorescent HDLs (red). Note the diffusion of HDLs in the telencephalic ventricle. (B) ApoA-I immunohistochemistry 3 hpi showing ApoA-I detection in the ventricular cavity and the ventricular zone. (C) ApoA-I immunohistochemistry showing ApoA-I detection (yellow) in GFAP::GFP-positive radial glial cells (NSCs in green) with DAPI cell nuclear counterstaining (blue). (D) PCNA-positive area quantification following PBS, plasmatic HDL (plHDL), and reconstituted HDL (rHDL) 24 h intracerebrovascular post-injection (n = 4–6 injected fish). No significant differences were observed between the groups. (E) Representative pictures of PCNA immunostaining 24 h post-injection with PBS, plasmatic HDL (plHDL), reconstituted HDL (rHDL). Scale bar = 800 μm (A), 150 μm (B), 21 μm (C), and 200 μm (E).