FIGURE

Fig. 4

ID

ZDB-FIG-210324-33

Publication

Olguin-Olguin et al., 2021 - Chemokine-biased robust self-organizing polarization of migrating cells in vivo

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Fig. 4

The role of contractility and of Ezrin function in ligand-independent self-organized polarization of PGCs. (A) Intracellular flow fields of cytoplasm and Esyt2a during PGC migration in wild-type embryos, measured using Bioflow software. White arrow indicates the direction of migration. (B) ROCK activity is required for the establishment of the rear. Lifeact is in magenta and Ezrin (Left), Esyt2a (Middle), and MTs (Clip170H, Right) are in green in cells expressing a DN-ROCK. (C) Enhanced contractility induced by expression of a constitutively active RhoA (CA-RhoA) results in hyperpolarization of the cell. Lifeact is in magenta and Ezrin (Left), Esyt2a, (Middle), and MTs (Clip170H, Right) are in green. Dashed line marks the contours of the cell front. (D) Hyperactive version of Ezrin (amino acids 1 to 583) is distributed over a larger portion of the cell perimeter (Left, Lifeact in magenta and Ezrin in green) and inhibits blebbing activity (graph on the Right, Ezrin (1 to 583) n is the number of cells, two-tailed t test with Welch’s correction, P = <0.0001). (Scale bars, 10 µm.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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