FIGURE 3

10-gingerol suppresses osteoclastogenesis in zebrafish osteoclastic scales. (A) Representative images for prednisolone (PN) treated regenerating zebrafish scales with 10-gingerol, GHE, or alendronate (AL; positive control). The lower panels show magnified images of the right lateral regions of the upper scales. Red color indicates the TRAP-positive area. The dark area in PN indicates the concentration of TRAP-positive cells. The black arrow indicates the resorbed edges, and white arrows show the resorption pits. For the experimental design of the zebrafish study, please see Supplementary Figure 2. (B) Quantitative analysis of (A). Integrative densities of the red areas were measured using ImageJ. n = 15–20, error bars indicate SE; ^∗∗p < 0.01 vs. PN. (C) Scales after calcein and alizarin red S staining. The upper panels show calcein staining, Alizarin red staining, and merged images, respectively. Scale bar = 500 μm.