Identification and validation of TULP3 protein–protein interactions.A, silver stained gel showing proteins coimmunoprecipitated alongside mock-FLAG or FLAG-TULP3 from 293T cells. The band corresponding to FLAG-TULP3 is indicated by a red arrow. B, bar graph ranking the top 35 high-confidence hits in (A) as identified by mass spectrometry. C, STRING diagram outlining the interconnectedness of TULP3 interactors identified in (B). Western blots showing coimmunoprecipitation of FLAG-TULP3 with (D) SIRT1, (E) RAD18, and (F) PP6R3 in 293T cells.
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