FIGURE 1

Pictorial representation of experimental procedure. The figure depicts the several time points (at day 1 and day 10) in the corresponding experimental groups where treatments were performed with VC, Pilo, PTZ, DZP and three doses of mAb. In day 1 and day 10, there is a recording of locomotor behavior for 15 min for each experimental group. On day 10, in the DZP groups and mAbs groups, pre-treatment was done 30 min prior to PTZ administration. Starting from day 10, T-maze test were performed for each group at the interval of 0h, 3h and 24 h. After 24 h T-maze test, all the fish were euthanized, and brain samples were collected which were further subjected to biochemical investigations. D1, Day 1; D10, Day 10, D11, Day 11; VC, Vehicle control (10% DMSO, I.P.); DMSO, dimethyl sulfoxide; I.P., Intraperitoneal; Pilo, Pilocarpine (400 mg/kg, I.P.); PTZ, Pentylenetetrazol (80 mg/kg, I.P.); DZP, Diazepam (1.25 mg/kg, I.P.); mAb 1, Anti-HMGB1 mAb (1 mg/kg, I.P.); mAb 2.5, Anti-HMGB1 mAb (2.5 mg/kg, I.P.); mAb 5, Anti-HMGB1 mAb (5 mg/kg, I.P.).