Immunofluorescence staining analysis of the retinal neuronal differentiation in the INL. All figures are horizontal sections along the temporal- nasal axis (T-N). (A–D″) Immunofluorescence staining with different ACs markers, GABA, Parvalbumin, Calretinin, and TH, in WT, CT, and MO retinas at 96 hpf. (a-d) statistical analysis of GABA+, Parvalbumin+, Calretinin+ and TH+ cells in WT, CT, and MO retinas at 96 hpf. Blue, DAPI staining of the nuclei. Scale bar=20 μm. (a–d) Statistical analysis of GABA+, Parvalbumin+, Calretinin+, and TH+ cells in WT (n=16), CT (n=15), and MO (n=16) retinas at 96 hpf. Results are represented as the mean±s.e.m. ns, P>0.05; assessed by one-way ANOVA followed by Tukey's multiple comparisons.
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