FIGURE

Figure 4

ID

ZDB-FIG-210310-68

Publication

Lu et al., 2021 - Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead

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Figure 4

Identification and functional characterization of zebrafish abcc4^−/− mutants. (A) PCR amplification of WT and mutant F₃ zebrafish. M: Molecular markers; WT: wild type zebrafish; F₃^+/−: heterozygous mutants. Red arrows point to the specific DNA bands for mutants. (B) Sequencing maps of WT and homozygous mutants. Oval frame: the 5-bp (CATGC) deletion in homozygotes. (C) The expression of Abcc4 protein in WT, abcc4^+/− and abcc4^−/− larvae at 96 hpf detected with Western blotting. (D) Survival rates of abcc4^−/− and WT larvae were monitored after exposure to 400 μM lead from 96 to 168 hpf. (E) Lead contents in abcc4^−/− and WT larvae after treatment with 10 μM lead at the indicated exposure time points. Values are expressed as means ± SD (n = 3). Significant differences are indicated by * p < 0.05 and ** p < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB abcc4^{ihb366/ihb366}
Condition:	chemical treatment by environment: lead(0)
Observed In:	detoxification of inorganic compound whole organism whole organism lead(0)
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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