ZFIN Image: Lu et al., 2021, Figure 4

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Figure Caption

Figure 4 Identification and functional characterization of zebrafish abcc4^−/− mutants. (A) PCR amplification of WT and mutant F₃ zebrafish. M: Molecular markers; WT: wild type zebrafish; F₃^+/−: heterozygous mutants. Red arrows point to the specific DNA bands for mutants. (B) Sequencing maps of WT and homozygous mutants. Oval frame: the 5-bp (CATGC) deletion in homozygotes. (C) The expression of Abcc4 protein in WT, abcc4^+/− and abcc4^−/− larvae at 96 hpf detected with Western blotting. (D) Survival rates of abcc4^−/− and WT larvae were monitored after exposure to 400 μM lead from 96 to 168 hpf. (E) Lead contents in abcc4^−/− and WT larvae after treatment with 10 μM lead at the indicated exposure time points. Values are expressed as means ± SD (n = 3). Significant differences are indicated by * p < 0.05 and ** p < 0.01.

Figure Data

Phenotype details

Acknowledgments

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