FIGURE

FIGURE 3

ID

ZDB-FIG-210310-26

Publication

Ranawakage et al., 2021 - Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor

Other Figures

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FIGURE 3

Effect of the lssDNA strand choice on knock-in efficiency. (A) Definition of the target and non-target strands in relation to the CRISPR-Cas9 complex. The strand that is complementary to the crRNA sequence is referred to as the target strand. (B) The target and non-target strands of lssDNA used as a donor template. Each lssDNA was microinjected with 1.5 fmol of the RNP complex into the cytoplasm of one-cell stage zebrafish embryos, and genomic DNA was extracted from 20-embryo pools. (C) Schematic illustration of the sox3 knock-in allele and knock-in allele-specific PCR. (D) Agarose gel image showing the PCR amplicons of knock-in allele-specific PCR at the 3′ junction of the integration and the β-actin2 gene-specific PCR (control) to confirm DNA integrity.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol