Fig. 5
- ID
- ZDB-FIG-210309-5
- Publication
- Pradhan et al., 2012 - Activation of NF-kappaB prevents the transition from juvenile ovary to testis and promotes ovarian development in zebrafish
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FIGURE 5. DOC activates NF?B and up-regulates p65 and ptgs2a expression in vitro. nZFL were exposed to DOC (200 ?m, 300 ?m) for 12 h, and luciferase activity was analyzed to check NF-?B activation (A). ZFL cells were exposed to DOC (200 ?m, 300 ?m) alone and combination (200 ?m DOC) with NAI (40 nm) for 24 h, and total RNA was extracted followed by qRT-PCR analysis of p65 (B and D) and ptgs2a (C and E) expression. The statistically significant difference between groups was determined using Student's t test (*, p < 0.05; **, p < 0.01; ***, p < 0.001). n = 4. Error bars represent the mean ± S.D. |