Fig. 3

FIGURE 3. The gonadal transcriptomes of individuals treated with heat-killed E. coli are similar to those of control females. Differential gene expression was determined by a homemade gonadal cDNA microarray in the differentiating zebrafish gonads at 35 dpf after treatment with an intermediate (Int; 5 × 10⁶ cfu/ml) and high dose (10⁷ cfu/ml) of heat-killed E. coli. A, shown is a visualization of the main source of experimental variation using principal components analysis of all normalized array expression values. B, shown is a Heatmap visualization of 1812 array features differentially expressed between the control male and control female samples (-fold change ≥ 2-fold; q value < 0.05). Rows were scaled to have a mean of zero and S.D. of one. C and D, shown is a Venn analysis of genes differentially expressed (-fold change ≥ 2-fold; q value < 0.05) between the two sexes from the E. coli treatment groups and the control samples, respectively. 1) gene sets are differentially expressed after treatment with intermediate dose of heat-killed bacteria. 2) gene sets are differentially expressed after treatment with high dose of heat-killed bacteria. CF, control female; CM, control male; H, high dose (10⁷ cfu/ml); I, intermediate dose (5 × 10⁶ cfu/ml). The number of genes that were not differentially expressed is indicated in the lower right corner of each panel.