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ADSC induce an EMT phenotype in SiHa cells. (a–d) Immunofluorescence analysis of EMT markers including fibronectin (a), Vimentin (b), N-cadherin (c) and E-cadherin (d) in SiHa cells cultured with or without conditioned medium of ADSC. EMT proteins were stained with Cy3-conjugated secondary antibody and nuclei were stained with 4′-6-Diamidino-2-phenolindole (DAPI). Images were taken in a confocal microscope using an × 40 oil lens. Photographs are representative of three independent experiments. To quantify the expression levels of fibronectin (e), vimentin (f), N-cadherin (g) and E-cadherin (h), the intensity of fluorescence was quantified using Image J. Graph represents three biological replicates, error bars are s.d. and **p < 0.01 and ***p < 0.001.
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