Fig. 4

Figure 4. Granulation Defects Are Also Found in tet2/3^MH Adult Neutrophils (A–D) Representative flow cytometry analysis of whole-kidney marrow (WKM) for 1-year-old wild-type, tet2^−/−, tet3^−/−, and tet2/3^MH zebrafish. Myeloid cells are delineated by the black circle, hematopoietic progenitors by a dark gray circle, lymphocytes by a medium gray circle, and red blood cells (RBCs) by a light gray circle. (E–H) Representative electron microscopy images of wild-type, tet2^−/−, tet3^−/−, and tet2/3^MH neutrophils isolated by FACS from kidneys of 1-year-old adult zebrafish. Red arrowheads indicate representative granules, blue arrowheads indicate autophagosomes, and the green arrowhead indicates nuclear envelope separation. (I) Quantification of the percentage of cells of each subtype: myeloid cells, progenitors, lymphocytes, and RBCs; n = 3/condition. (J) Histogram comparing FSC-A for the myeloid gate in each genotype for a representative sample. (K) Quantification of mean FSC-A for the myeloid gate in each genotype; n = 3/condition. (L) Quantification of granules per cell in electron microscopy images; n = 4/condition. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 compared to wild type by one-way ANOVA with Tukey post hoc test.