FIGURE

Figure 4

ID

ZDB-FIG-210227-9

Publication

Yang et al., 2021 - Ca²⁺-Dependent Glucose Transport in Skeletal Muscle by Diphlorethohydroxycarmalol, an Alga Phlorotannin: In Vitro and In Vivo Study

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Figure 4

Detection of cytosolic Ca²⁺ changes using the Fluo-4 in zebrafish larvae. (a) Zebrafish larvae were stimulated with only DPHC (1.2, 6, and 12 μM). (b) Zebrafish larvae were stimulated with DPHC in Fluo-4 and compared with/without Fluo-4 groups. Changes in cytosolic Ca²⁺ levels were measured by changes in fluorescence intensity of Fluo-4 using ImageJ. (c) Measurement of blood glucose level in zebrafish. Zebrafish were injected with BAPTA-AM (3 μg/g body weight) for 1 h, after which the zebrafish were injected with DPHC (0.3 μg/g body weight) for 90 min. Experiments were performed in triplicate, and the data are expressed as the mean± SE, n = 4 per group. *,^#Values having different superscripts are significantly different at ****p<0.0001 compared with the no sample-treated group; ^##p<0.01 and ^####p<0.0001 compared with the nontreated group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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