Fig. 1

Unbiased MIR screening and confirmation in the endothelium of mice with a systemic inflammatory response. Mice were either given LPS (17.5 mg/kg BW i.p.) or vehicle (0.9% NaCl) and killed after 24 h or at indicated time-points. a A smallRNA sequencing was conducted using isolated CD146 + pulmonary endothelial cells (ECs) from endotoxemic compared to healthy mice. Shown is a heat map that highlights the gene distribution with the arrow pointing to the upregulation of MIR155. b Volcano plot showing a log scaled distribution of 318 analyzed endothelial MIRs. 5 MIRs were down- and 31 upregulated; MIR155 (arrow) showed the strongest upregulation (c). Bar graphs showing normalized MIR155 expression in lung lysates via RT-PCR after 4, 12 and 24 h compared to control (CTR) (CTR: n = 8; LPS: n = 4–5). d Bar graphs showing normalized MIR155/Rnu6b expression in CD146 + pulmonary ECs (n = 5). e In vitro confirmation of time-dependent MIR155 upregulation in human umbilical endothelial cells (HUVEC) (n = 4–6) after stimulation with the proinflammatory cytokine TNFa (50 ng/mL) compared to vehicle (CTR); Bar graphs show mean ± SD of normalized MIR155 with a maximum of 15 × fold increase at 24 h. (c–e, all **p < 0.01)