Fig. 5

Fig. 5. Recombinant zfIFNϕ1-induced changes in the expression of mxa gene. The mxa gene expression is normalized against the housekeeping gene rps11. Changes in mxa expression in 5 pooled larvae of zebrafish injected with recombinant zfIFN1 at 2.5 dpf (striped bars) are shown as x-fold increase compared to PBS injected control larvae (white bars) in each time point (A). The symbol (*) indicates significant differences between the zfIFNϕ1 stimulated and non-stimulated larvae at each time point (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) as revealed by two-way ANOVA followed by a Bonferroni test. Each bar represents the mean + SD of n = 6 samples. Changes in mxa expression in 5 pooled larvae of zebrafish injected with PBS (white bars) or recombinant zfIFNϕ1 (black bars) into the duct of Cuvier at 2.5 dpf (B). Larvae were infected with TiLV by injection into the duct of Cuvier 6 h post zfIFNϕ1 or PBS treatment. The symbol (*) indicates significant differences between the zfIFNϕ1 treated (24 and 48 hpi) or PBS treated (48 hpi) larvae and PBS treated larvae at 24 hpi (*p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001) as revealed by one-way ANOVA followed by Dunnett multiple comparison test. Each bar represents the mean + SD of n = 9–10 samples derived from two independent experiments.