Fig. 3

Fig. 3. DHA-S evoked mitochondrial Ca²⁺ accumulated in H9c2 cells. A, Dynamic change of cytoplasm Ca²⁺ was determined by Fluo3-AM. Right, quantification of the Fluo3 fluorescence intensity before and after adding Ca²⁺ (5?mM). Data were analyzed by one-way analysis of variance (ANOVA) test; F (3, 8)?=?33.8 and F (3, 8)?=?20.97 for the base and post Ca²⁺, respectively. H9c2 treated with the DHA-S (1?g/L, about 5?mM) in EBSS. BAPTA-AM and ionomycin were utilized as negative and positive control, respectively. B, Dynamic change of mitochondrial Ca²⁺ was determined by Rhod2-AM. Right, quantification of the Rhod2 fluorescence intensity before and after adding Ca²⁺ (5?mM). Data were analyzed by one-way analysis of variance (ANOVA) test; F (3, 8)?=?4.163 and F (3, 8)?=?6.465 for the base and post Ca²⁺, respectively. C, Represented images of H9c2 cells, after treated with DHA-S (5?g/L, 25?mM) for 24?h, labeled with Rhod-2 AM and Mito-Tracker Green. Scale bar, 10?µm. D, Quantification of the Rhod-2-positive cells in P1 gate by FACS and the results showed as histograms (down). Data were analyzed by Student?s unpaired T test. All experiments were repeated three times and the data were expressed as mean ±?SEM. *, P?