PUBLICATION

Sodium dehydroacetate induces cardiovascular toxicity associated with Ca2+ imbalance in zebrafish

Authors
Huang, X., Zhao, X., Zhu, K., Ding, S., Shao, B.
ID
ZDB-PUB-210106-23
Date
2021
Source
Ecotoxicology and environmental safety   208: 111613 (Journal)
Registered Authors
Keywords
Ca(2+), Cardiovascular, Metabolism, Mitochondria, Sodium dehydroacetate
MeSH Terms
  • Calcium/metabolism*
  • Myocardium/metabolism
  • Myocardium/pathology
  • Edema, Cardiac/chemically induced
  • Embryonic Development/drug effects*
  • Embryo, Nonmammalian/drug effects*
  • Heart/drug effects*
  • Heart/embryology
  • Zebrafish*/growth & development
  • Cerebral Hemorrhage/chemically induced
  • Dose-Response Relationship, Drug
  • Cell Line
  • Animals
  • Pericardium/drug effects
  • Pericardium/pathology
  • Apoptosis/drug effects
  • Pyrones/toxicity*
  • Cardiotoxicity
  • Rats
(all 19)
PubMed
33396133 Full text @ Ecotoxicol. Environ. Saf.
Abstract
The environmental effects of additives have attracted increasing attention. Sodium dehydroacetate (DHA-S), as an approved preservative, is widely added in processed foods, cosmetics and personal care products. However, DHA-S has been recently reported to induce hemorrhage and coagulation aberration in rats. Yet little is known about the ecotoxicological effect and underlying mechanisms of DHA-S. Here, we utilized the advantage of zebrafish model to evaluate such effects. DHA-S induced cerebral hemorrhage, mandibular dysplasia and pericardial edema in zebrafish after 24 h exposure (48-72 hpf) at 50 mg/L. We also observed the defective heart looping and apoptosis in DHA-S-treated zebrafish through o-dianisidine and acridine orange staining. Meanwhile, DHA-S induced the deficiency of Ca2+ and vitamin D3 in zebrafish. We further demonstrated that DHA-S stimulated Ca2+ influx resulting in Ca2+-dependent mitochondrial damage in cardiomyocytes. Additionally, DHA-S inhibited glucose uptake and repressed the biosynthesis of amino acids. Finally, we identified that sodium bicarbonate could rescue zebrafish from DHA-S induced cardiovascular toxicity. Altogether, our results suggest that DHA-S is a potential risk for cardiovascular system.
Genes / Markers
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Orthology
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Engineered Foreign Genes
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Citation
Huang, X., Zhao, X., Zhu, K., Ding, S., Shao, B. (2021) Sodium dehydroacetate induces cardiovascular toxicity associated with Ca2+ imbalance in zebrafish. Ecotoxicology and environmental safety. 208:111613.