FIGURE

Fig. 3

ID

ZDB-FIG-210217-58

Publication

Turan et al., 2020 - The endosomal trafficking regulator LITAF controls the cardiac Nav1.5 channel via the ubiquitin ligase NEDD4-2

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Fig. 3

Figure 3. Physical interaction between LITAF and Nav1.5 in HEK cells and 3wRbCM. A, V5 IP of lysates from HEK cells stably expressing V5-tagged Nav1.5 and transfected with plasmids for HA-tagged LITAF or empty expression plasmid (control) (n = 3). The right panel shows immunoprecipitated Nav1.5 and co-precipitated LITAF-HA and thus an interaction between LITAF and Nav1.5 (the asterisk indicates the light chain of the IP capture antibody). Input levels of Nav1.5 and LITAF-HA are shown in the left panel. B, Duolink in situ proximity ligation assay using mouse anti-LITAF and rabbit anti-Nav1.5 antibodies in 3wRbCM. The co-localization between molecules is indicated by red puncta (left panel). Virtually no puncta were detected in negative controls in which only one antibody was used, i.e. rabbit anti-Nav1.5 (middle panel) or mouse anti-LITAF antibodies (right panel). The nuclei were stained with DAPI (blue). Depicted merged confocal images (bright field, DAPI, and Texas Red) are representative of each condition. Scale bar, 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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