FIGURE

Fig. 1

ID

ZDB-FIG-210217-49

Publication

Begeman et al., 2020 - Decoding an Organ Regeneration Switch by Dissecting Cardiac Regeneration Enhancers

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Fig. 1

Regeneration-dependent activity of cardiac LEN (cLEN). (A) Schematic of lepb regeneration enhancer (LEN) containing two tissue-specific regeneration modules. A transgene construct examined for cLEN activity consists of 317?bp of cLEN, lepb 2?kb upstream promoter (P2) and EGFP reporter sequences. (B) Schematic of cLEN annotated with predicted TF binding sites. (C-F) Zebrafish strain and experimental strategy undertaken to ablate CMs (C) and images of uninjured and ablated hearts in larvae (D), juveniles (E) and adults (F). (G-I) Dynamic cLEN activity during zebrafish heart regeneration. (G) Experimental design to determine cLEN activity during heart regeneration. (H) Quantification of EGFP expression intensity normalized to images of wild-type uninjured hearts at the wound area. The data are presented as the mean�s.d. Numbers of animals are shown in Table S3. *P<0.05; **P<0.01; N.S., not significant. One-way ANOVA with Tukey's post-hoc test. (I) Images of cardiac sections of uninjured and regenerating hearts. Scale bars: 100?�m in E?,F?,I; 50?�m in D?.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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