Fig. 1
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- ZDB-FIG-210216-42
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- Trivellin et al., 2020 - The X-linked acrogigantism-associated gene gpr101 is a regulator of early embryonic development and growth in zebrafish
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Fig. 1. gpr101/GPR101 exogenous expression experiments. a) WT embryos were injected with 75 pg of mRNA and scored at 1 dpf. About 40% of embryos showed a mutant phenotype after either gpr101 or GPR101 injection. Injection with eGFP mRNA resulted in 24% of embryos with non-specific phenotypes. The total number of analyzed embryos is shown at the top of each column. Data shown are from one representative experiment. For each experiment, two expert operators performed injections. b) The two main phenotypes observed in gpr101/GPR101 injected embryos – cyclopia and dorsalization – are shown, together with their frequency. The white arrowheads indicate the fused eyes. Upper panels: ventral view; lower panels: lateral view. Anterior is left. Scale bar: 200 μm. c) Growth trajectories in eGFP (controls, black), gpr101 (orange) and GPR101 (light blue) injected embryos. Body surface was measured in larvae at 2 (eGFP, n = 17; gpr101, n = 21; GPR101, n = 20), 3 (eGFP, n = 17; gpr101, n = 21; GPR101, n = 19), and 4 (eGFP, n = 16; gpr101, n = 19; GPR101, n = 19) wpf (left panel), while standard length and body weight were measured in 7 wpf juvenile fish (eGFP, n = 16; gpr101, n = 18; GPR101, n = 19; right panel). No significant differences in growth among the three groups were observed at any of the analyzed time points. |
Reprinted from Molecular and Cellular Endocrinology, 520, Trivellin, G., Tirosh, A., Hernández-Ramírez, L.C., Gupta, T., Tsai-Morris, C.H., Faucz, F.R., Burgess, H.A., Feldman, B., Stratakis, C.A., The X-linked acrogigantism-associated gene gpr101 is a regulator of early embryonic development and growth in zebrafish, 111091, Copyright (2020) with permission from Elsevier. Full text @ Mol. Cell. Endocrinol.