FIGURE

Fig. 7

ID

ZDB-FIG-210215-16

Publication

Werner et al., 2021 - Hallmarks of primary neurulation are conserved in the zebrafish forebrain

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Fig. 7

Oscillatory constriction with decreasing amplitude reduces the apical surface of MHP cells.

a Measurements of medial, MHP cells. a1 Relative apical surface areas over time for individual MHP cells. a2 Median values of MHP relative apical surface areas over time, 95% confidence interval, n = 71. a3 Representative trace of relative apical surface area over time for an individual MHP cell. b Measurements of MHP-adjacent cells. b1 Relative apical surface areas over time for individual MHP-adjacent cells. b2 Median values of MHP-adjacent relative apical surface areas over time, 95% confidence interval. b3 Representative trace of relative apical surface area over time for an individual MHP-adjacent cell. c Distributions of the duration of oscillation between two expanded states for MHP cells (red, median of 45 s per oscillation) and MHP-adjacent cells (blue, median of 45 s per oscillation). No significant difference (two-sided Mann–Whitney U test, P = 0.293, AUC = 0.489, n = 758 MHP cell oscillations, n = 1,113 MHP-adjacent oscillations). d Distributions of the timing of apical constrictions or expansions for MHP cells (red) and MHP-adjacent cells (blue). There is no significant difference between the two groups for constriction time (two-sided Mann–Whitney U test: constrictions, P = 0.541, AUC = 0.510, n = 458 MHP cell constrictions, n = 640 MHP-adjacent constrictions) but there is a statistically significant difference for expansion time (P = 0.000832, AUC = 0.441, n = 367 MHP cell expansions, n = 596 MHP-adjacent expansions). The median time for individual expansions of MHP and MHP-adjacent cells is 15 and 30 s, respectively. All boxplot elements depict quartiles with the centerline depicting the median. e Still frames of time-lapse movie of mGFP-labeled cells shown in greyscale. The oscillatory behavior of one cell, outlined in red, is shown over time. Scale bar: 10 μm in e.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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