FIGURE

Fig. 1

ID

ZDB-FIG-210208-55

Publication

Zaksauskaite et al., 2021 - Tdp1 protects from topoisomerase 1-mediated chromosomal breaks in adult zebrafish but is dispensable during larval development

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Fig. 1

Zebrafish Tdp1 protein and its expression.

(A) Schematic structure of zebrafish Tdp1 protein, depicting the nuclear localization signal (NLS), phosphodiesterase domain, and active site histidines H270 and H501 (64). Zebrafish H501 corresponds to human H493. (B) Alignment of Homo sapiens (hs) and D. rerio (dr) TDP1 protein sequence. Identical sequences are highlighted in black, and similar sequences are highlighted in gray. The sequence targeted for deletion and catalytic HKD motifs [HxK(x)₄D(x)₆GSxN] with catalytic sites (in red) are noted (64, 65). Yellow denotes amino acids that contact the DNA substrate during repair. Accession numbers are as follows: hsTDP1, ENST00000335725.8, and drTdp1, ENSDART00000150149.2. Sequences were aligned using the Protein BLAST website: https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins. (C) Whole-mount in situ hybridization for tdp1 mRNA in wild-type zebrafish at 24 hpf. Sense probes for tdp1 mRNA were used as a negative control.

Expression Data

Gene:	tdp1
Fish:	WT
Anatomical Terms:	head whole organism
Stage:	Prim-5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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