Figure 4

Derazantinib interferes with endothelial cell junctions. Confocal images of GFP+ endothelial cell junctions and mCherry+ endothelial cell nuclei in Tg(fli1:pecam1-EGFP)^ncv27/Tg(fli1:NLS-mCherry)^ubs10 transgenic embryos from 38 hpf after treatment with DMSO (control, a) or DZB (b and c). a’–c’ depict only the GFP+ channel. Arrowheads point to multicellular continuous cell junctions, indicative in the control group (DMSO, a and a’), while arrows point to linear discontinuous junctions, indicative in the DZB-treated embryos (b and c’). The asterisk marks a sprouting defect (b and b’). (d) Quantification of ratio of continuous cell junctions to linear discontinuous junctions in ISVs per embryo (n ≥ 15 embryos per treatment were analysed from three independent experiments). Data in d represent mean ± S.E.M. (error bars), * p < 0.05, *** p < 0.001. Statistical analysis was performed with the two-sided Mann–Whitney test. Scale bar, 20 µm. See also Figure S3.