Figure 6

Alcian blue images for nectin1a zebrafish mutant compared to wild type at day 5. Top images: wild type alcian blue lateral view, palate and Meckel’s cartilage. Middle images: heterozygous nectin1a embryo alcian blue. Bottom images: homozygous nectin1a mutant lateral view. The length of the palate was measured from the anterior midpoint to the posterior midpoint of the palate. The width was measured as the maximum distance between the 2 lateral borders at the anterior area. The length of the Meckel’s cartilage was measured from the midline of the Meckel’s cartilage to the midline of an imaginary line drawn joining the joints between the Meckel’s cartilage and the palatoquadrate. The width was measured from the junction of the Meckel’s cartilage and the palatoquadrate of one side to the other side. Compared to wild type animals. nectin1a mutants have smaller and shorter palate, and shorter and wider Meckel’s cartilage. L length, W width. Scale bar: 10 μm.