FIGURE

Fig. 1

ID

ZDB-FIG-210112-4

Publication

Giarmarco et al., 2020 - Daily mitochondrial dynamics in cone photoreceptors

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Fig. 1

At night single cones have more small mitochondria. (A) Schematic of zebrafish cone subtypes (Left), with EM (Middle) and IHC (Right) images of zebrafish outer retina. IHC images are stained for all mitochondria (green), red-green cone OSs (magenta), and nuclei (blue). Arrowheads indicate corresponding UV, blue, green, and red cone mitochondrial clusters; white arrows, megamitochondria at UV cluster bases. Yellow box, zoomed-in area. (Scale bars, 10 µm.) (B) Example 50-nm Z-stack from SBFSEM used for 3D analysis via manual segmentation or rapid dot quantification. (C) Z-stacks from SBFSEM with 3D-rendered mitochondrial clusters (colored) and OSs (gray) from green, blue, and UV cone subtypes in daytime at ZT6 (15:00) and night at ZT16 (01:00). (Scale bars, 5 µm.) (D) Violin plots of mitochondrial number, cluster volume, and mitochondrial volume from dot quantification and manual segmentation; lines represent median. Cone subtypes are represented by respective colors at ZT6 (circles) and ZT16 (squares). *,‡P < 0.05. **, ‡‡P < 0.01. ***,‡‡‡P < 0.001. SI Appendix, Table S2 lists statistical information and Ns from each group.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	constant dark
Observed In:	long single cone cell mitochondrion short double cone cell mitochondrion short single cone cell mitochondrion
Stage:	Adult

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA