PUBLICATION

Daily mitochondrial dynamics in cone photoreceptors

Authors
Giarmarco, M.M., Brock, D.C., Robbings, B.M., Cleghorn, W.M., Tsantilas, K.A., Kuch, K.C., Ge, W., Rutter, K.M., Parker, E.D., Hurley, J.B., Brockerhoff, S.E.
ID
ZDB-PUB-201120-24
Date
2020
Source
Proceedings of the National Academy of Sciences of the United States of America   117: 28816-28827 (Journal)
Registered Authors
Brockerhoff, Susan, Hurley, James B., Parker, Ed
Keywords
circadian, mitochondria, photoreceptors, retina, zebrafish
MeSH Terms
  • Endoplasmic Reticulum/metabolism
  • Synapses/metabolism
  • Retinal Cone Photoreceptor Cells/metabolism*
  • Animals
  • Retina/metabolism
  • Mitochondria/metabolism*
  • Dark Adaptation/physiology
  • Zebrafish
  • Circadian Rhythm/physiology
  • Mitochondrial Dynamics/physiology*
(all 10)
PubMed
33144507 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Cone photoreceptors in the retina are exposed to intense daylight and have higher energy demands in darkness. Cones produce energy using a large cluster of mitochondria. Mitochondria are susceptible to oxidative damage, and healthy mitochondrial populations are maintained by regular turnover. Daily cycles of light exposure and energy consumption suggest that mitochondrial turnover is important for cone health. We investigated the three-dimensional (3D) ultrastructure and metabolic function of zebrafish cone mitochondria throughout the day. At night retinas undergo a mitochondrial biogenesis event, corresponding to an increase in the number of smaller, simpler mitochondria and increased metabolic activity in cones. In the daytime, endoplasmic reticula (ER) and autophagosomes associate more with mitochondria, and mitochondrial size distribution across the cluster changes. We also report dense material shared between cone mitochondria that is extruded from the cell at night, sometimes forming extracellular structures. Our findings reveal an elaborate set of daily changes to cone mitochondrial structure and function.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WTconstant darkAdult
WTconstant darkAdult
WTconstant darkAdult
WTconstant darkAdult
WTconstant darkAdult
WTconstant darkAdult
WTconstant darkAdult
WTconstant darkAdult
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
a9
    		Complex
    w138TgTransgenic Insertion
      w140TgTransgenic Insertion
        w204TgTransgenic Insertion
          w258
            		Unknown
            1 - 5 of 5
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            Human Disease / Model
            No data available
            Sequence Targeting Reagents
            No data available
            Fish
            Fish
            WT
            1 - 1 of 1
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            Antibodies
            No data available
            Orthology
            Engineered Foreign Genes
            Marker Marker Type Name
            cpYFPEFGcpYFP
            GFPEFGGFP
            1 - 2 of 2
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            Mapping
            No data available
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            Citation
            Giarmarco, M.M., Brock, D.C., Robbings, B.M., Cleghorn, W.M., Tsantilas, K.A., Kuch, K.C., Ge, W., Rutter, K.M., Parker, E.D., Hurley, J.B., Brockerhoff, S.E. (2020) Daily mitochondrial dynamics in cone photoreceptors. Proceedings of the National Academy of Sciences of the United States of America. 117:28816-28827.